Part:BBa_K4623004:Design
Basic Silinker (TrxA-His-thrombin-mSA-SBP)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 445
Illegal AgeI site found at 505 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is appended with an His tag, allowing for purification using a nickel column.
Source
It is a new fusion protein we have designed. We combined the sequence of Trx-His-thrombin-mSA-SBP.
References
[1]Sano, T., & Cantor, C. R. (1990). Expression of a cloned streptavidin gene in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America, 87 (1), 142–146.
[2]Lim, K. H., Huang, H., Pralle, A., & Park, S. (2011). Engineered streptavidin monomer and dimer with improved stability and function. Biochemistry, 50(40), 8682–8691.
[3] Demonte, D., Dundas, C. M., & Park, S. (2014). Expression and purification of soluble monomeric streptavidin in Escherichia coli. Applied microbiology and biotechnology, 98 (14), 6285–6295.